This new volume provides a bird’s-eye view of the properties, utilization, and importance of high resolution mass spectrometry (HRMS) for phytochemical analysis. The book discusses the new and state-of-the-art technologies related to HRMS in phytochemical analysis for the food industry in a comprehensive manner. Phytochemical characterization of plants is important in the food and nutraceutical industries and is also necessary in the procedures followed for drug development, toxicology determination, forensic studies, origin verification, quality assurance, etc. Easy determination of active compounds and isolation as well as purification of the same from natural matrices are required, and the possibilities and advantages of HRMS pave the way for improved analysis patterns in phytochemistry. This book is unique in that its sole consideration is on the importance of HRMS in the field of phytochemical analysis. Along with an overview of basic instrumental information, the volume provides a detailed account of data processing and dereplication strategies. Technologies such as bioanalytical techniques and bioassays are considered also to provide support for the functions of the instruments used. In addition, a case study is presented to depict the complete phytochemical characterization of a matrix by HRMS. The book covers processing and computational techniques, dereplication, hyphenation, high-resolution bioassays, bioanalytical screening/purification techniques, applications of gas chromatography–high-resolution mass spectrometry, and more. Key features: Covers the fundamental instrumentation and techniques Discusses HRMS-based phytochemical research details Focuses strictly on the phytochemical considerations High-Resolution Mass Spectroscopy for Phytochemical Analysis: State-of-the-Art Applications and Techniques will be a valuable reference guide and resource for researchers, faculty and students in related fields, as well as those in the phytochemical industries.

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp

This new volume provides a bird’s-eye view of the properties, utilization, and importance of high resolution mass spectrometry (HRMS) for phytochemical analysis. The book discusses the new and state-of-the-art technologies related to HRMS in phytochemical analysis for the food industry in a comprehensive manner. Phytochemical characterization of plants is important in the food and nutraceutical industries and is also necessary in the procedures followed for drug development, toxicology determination, forensic studies, origin verification, quality assurance, etc. Easy determination of active compounds and isolation as well as purification of the same from natural matrices are required, and the possibilities and advantages of HRMS pave the way for improved analysis patterns in phytochemistry. This book is unique in that its sole consideration is on the importance of HRMS in the field of phytochemical analysis. Along with an overview of basic instrumental information, the volume provides a detailed account of data processing and dereplication strategies. Technologies such as bioanalytical techniques and bioassays are considered also to provide support for the functions of the instruments used. In addition, a case study is presented to depict the complete phytochemical characterization of a matrix by HRMS. The book covers processing and computational techniques, dereplication, hyphenation, high-resolution bioassays, bioanalytical screening/purification techniques, applications of gas chromatography–high-resolution mass spectrometry, and more. Key features: Covers the fundamental instrumentation and techniques Discusses HRMS-based phytochemical research details Focuses strictly on the phytochemical considerations High-Resolution Mass Spectroscopy for Phytochemical Analysis: State-of-the-Art Applications and Techniques will be a valuable reference guide and resource for researchers, faculty and students in related fields, as well as those in the phytochemical industries.

Applications of High Resolution Mass Spectrometry: Food Safety and Pesticide Residue Analysis is the first book to offer complete coverage of all aspects of high resolution mass spectrometry (HRMS) used for the analysis of pesticide residue in food. Aimed at researchers and graduate students in food safety, toxicology, and analytical chemistry, the book equips readers with foundational knowledge of HRMS, including established and state-of-the-art principles and analysis strategies. Additionally, it provides a roadmap for implementation, including discussions of the latest instrumentation and software available. Detailed coverage is given to the application of HRMS coupled to ultra high-performance liquid chromatography (UHPLC-HRMS) in the analysis of pesticide residue in fruits and vegetables and food from animal origin. The book also discusses extraction procedures and the challenges of sample preparation, gas chromatography coupled to high resolution mass spectrometry, flow injection-HRMS, ambient ionization, and identification of pesticide transformation products in food. Responding to the fast development and application of these new procedures, this book is an essential resource in the food safety field. Arms researchers with an in-depth resource devoted to the rapid advances in HRMS tools and strategies for pesticide residue analysis in food Provides a complete overview of analytical methodologies and applications of HRMS, including UHPLC-HRMS, HRMS coupled with time of flight (TOF) and/or GC-Orbitrap, and flow injection-HRMS Discusses the current international regulations and legislation related to the use of HRMS in pesticide residue analysis Features a chapter on the hardware and software available for HRMS implementation Offers separate chapters on HRMS applied to pesticide residue analysis in fruits and vegetables and in food from animal origin

For graduate students and professional workers in the biochemical, biological, agricultural, and biomedical sciences.

This volume contains reviews which are based on a symposium, given th at the 30 meeting of The Phytochemical Society of North America, held at Laval University in Quebec City, Canada on August 11-15, 1990. During the past two decades, there have been major new developments in methods which can be applied toward the isolation, separation and structure determination of complex natural products. Therefore, the topic of this symposium, "Modem Phytochemical Methods", is a very timely one. The organizers of the symposium recognized that it would not be possible to cover in detail all new advances in phytochemical methodology. It was therefore decided to emphasize general reviews on recent developments of major separation techniques such as high performance liquid chromatography as well as supercritical fluid chromato graphy. In addition, advances in commonly used structure determination methods, mainly NMR and MS, are reviewed. Other topics include methodo logies of micro-sampling for isolation and analysis of trichome constituents as well as recent breakthroughs on biosynthetic studies of monoterpenes using "enriched" basal cells of trichomes. The volume concludes with a review of quantitative structure-activity relationship (QSAR) studies of biologically active natural products. In Chapter I, K. Hostettmann and his colleagues give a general review of recent developments in the separation of natural products with major emphasis on preparative separations of biologically active plant constituents. The authors present a comparison of droplet countercurrent chromatography (OCCC) with the highly rapid and more versatile centrifugal partition chromatography (CPC).