Fluorescence Microscopy of Living Cells in Culture, Part B

Fluorescence Microscopy of Living Cells in Culture, Part A

Parts A and B, companion volumes on fluorescence microscopy, are sourcebooks of methods for living cells. Part A comprehensively covers methodology: fluorescent analogs, procedures for fluorescent labeling of various cellular structures, and fluorescence microscopy. Part B describes instrumentation and quantitation analysis for imaging and spectroscopy. Key Features * Convenient bench-top format Comprehensive methods inluded for: * Tracing proteins, peptides, hormones, and toxins * Labeling cell surfaces, mitochondria, endoplasmic reticulum, and endocytic compartments * Preparation of fluorescent probes and their incorporation into cells * Culturing cells under the microscope * Basic fluorescence microscopy and imaging techniques

Only recently has the true power of fluorescence techniques evolved for use with single living cells. The present status of the field reflects the occurrence of a revolution in cell biological research. Volume 30, along with Volume 29, provide the cell biologist with a sourcebook of methods. Volume 29 deals with the preparation, delivery, and detection of fluorescent probes. Volume 30 explores a combination of the theoretical and technical issues related to the quantitation of fluorescence signals in the living cell with a light microscope.