Voltage and Patch Clamping with Microelectrodes
Title | Voltage and Patch Clamping with Microelectrodes PDF eBook |
Author | Thomas G Smith |
Publisher | Springer |
Total Pages | 263 |
Release | 2013-05-27 |
Genre | Medical |
ISBN | 1461476011 |
Edited and authored by international experts on voltage and patch clamping, this volume is designed to help anyone undertaking experiments requiring the use of these techniques. The only book of its kind to bring together this wealth of information on theory as well as practical techniques, this is a volume that no one involved in voltage and patch clamping can afford to be without.
Patch Clamping
Title | Patch Clamping PDF eBook |
Author | Areles Molleman |
Publisher | John Wiley & Sons |
Total Pages | 186 |
Release | 2003-06-13 |
Genre | Science |
ISBN | 0470856513 |
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
Patch-Clamp Analysis
Title | Patch-Clamp Analysis PDF eBook |
Author | Wolfgang Walz |
Publisher | Springer Science & Business Media |
Total Pages | 349 |
Release | 2008-02-06 |
Genre | Medical |
ISBN | 1592592767 |
Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.
Patch Clamp Techniques
Title | Patch Clamp Techniques PDF eBook |
Author | Yasunobu Okada |
Publisher | Springer |
Total Pages | 0 |
Release | 2012-04-27 |
Genre | Medical |
ISBN | 9784431539926 |
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Microelectrode Techniques
Title | Microelectrode Techniques PDF eBook |
Author | David Ogden |
Publisher | |
Total Pages | 474 |
Release | 1994 |
Genre | Science |
ISBN |
Single-Channel Recording
Title | Single-Channel Recording PDF eBook |
Author | Bert Sakmann |
Publisher | Springer Science & Business Media |
Total Pages | 506 |
Release | 2013-11-11 |
Genre | Medical |
ISBN | 1461578582 |
Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.
Manual of Research Techniques in Cardiovascular Medicine
Title | Manual of Research Techniques in Cardiovascular Medicine PDF eBook |
Author | Hossein Ardehali |
Publisher | John Wiley & Sons |
Total Pages | 1 |
Release | 2013-12-16 |
Genre | Medical |
ISBN | 1118495136 |
While some research methods or techniques are applicable in several areas of medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques and measurements that are highly specialized and unique to this area of investigation. Edited by senior clinical investigators who are recognized leaders in cardiovascular medicine worldwide, this book provides readers with a comprehensive, practical “how-to-do-it” review of best-practice techniques for cardiovascular research.