Understanding what drives popular culture is crucial for the church - whether we are consumers or creators. It will help us relate to the stories, the poetry, the idolatry of our times - and so to speak powerfully to our culture's hopes and fears.

Themelios is an international, evangelical, peer-reviewed theological journal that expounds and defends the historic Christian faith. Themelios is published three times a year online at The Gospel Coalition (http://thegospelcoalition.org/themelios/) and in print by Wipf and Stock. Its primary audience is theological students and pastors, though scholars read it as well. Themelios began in 1975 and was operated by RTSF/UCCF in the UK, and it became a digital journal operated by The Gospel Coalition in 2008. The editorial team draws participants from across the globe as editors, essayists, and reviewers. General Editor: D. A. Carson, Trinity Evangelical Divinity School Managing Editor: Brian Tabb, Bethlehem College and Seminary Consulting Editor: Michael J. Ovey, Oak Hill Theological College Administrator: Andrew David Naselli, Bethlehem College and Seminary Book Review Editors: Jerry Hwang, Singapore Bible College; Alan Thompson, Sydney Missionary & Bible College; Nathan A. Finn, Southeastern Baptist Theological Seminary; Hans Madueme, Covenant College; Dane Ortlund, Crossway; Jason Sexton, Golden Gate Baptist Seminary Editorial Board: Gerald Bray, Beeson Divinity School Lee Gatiss, Wales Evangelical School of Theology Paul Helseth, University of Northwestern, St. Paul Paul House, Beeson Divinity School Ken Magnuson, The Southern Baptist Theological Seminary Jonathan Pennington, The Southern Baptist Theological Seminary James Robson, Wycliffe Hall Mark D. Thompson, Moore Theological College Paul Williamson, Moore Theological College Stephen Witmer, Pepperell Christian Fellowship Robert Yarbrough, Covenant Seminary

The richly varied phenomenon of urban popcultures, through distinctive practices and forms, has significantly marked the life of modern city.

This volume has as its central theme the presentation of original papers which seek to critique, deconstruct and go beyond existing research and knowledge frontiers in tourism. The text also includes debates on the value of tourism research at the institutional level and discussions of tourism research agendas which still remain under or unexplored

Business and Buddhism explores alternative ways of leading in the aftermath of the Great Recession and the many stories of fraud and greed that emerged. The book explores shifts in business perspectives as more value is placed on soft skills like emotional intelligence and listening, and introduces the reader to the principles in Buddhist philosophy that can be applied in the workplace. Buddhist practices are increasingly understood as spiritual, rather than religious per se. In fact, Buddhism is alternately referred to as a philosophy or psychology. In this book, Marques explores the value of applying the positive psychology of Buddhism to work settings. She outlines the ways in which it offers highly effective solutions to addressing important management and organizational behavior related issues, but also flags up critical areas for caution. For example, Buddhism is non-confrontational, and promotes detachment. How can business leaders negotiate these principles in light of the demands of modern day pressures? The book includes end of chapter questions to promote reflection and critical thinking, and examples of Buddhist leaders in action. It will prove a captivating read for students of organizational behavior, management, leadership, diversity and ethics, as well as business consultants.

1. Expression strategy (Michael Dyson) 2. Protein expression in Escherichia coli (Rosalind Kim) 3. Expression engineering of synthetic antibodies using ribosome display (Matthew DeLisa and Lydia M. Contreras Martinez) 4. Refolding proteins from inclusion bodies (Renaud Vincentelli) 5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters (Geoffrey Waldo and Stéphanie Cabantous) 6. Protein expression in the wheat germ cell-free system (Yaeta Endo and Tatsuya Sawasaki) 7. Saccharomyces cerevisiae ; A microbial eukaryotic expression system (Christine Lang) 8. Expression of proteins in Pichia pastoris (Geoff and Joan Lin-Cereghino and Wilson Leung) 9. Improved baculovirus expression vectors (Linda King, Richard Hitchman and Robert Possee) 10. Transient transfection of insect cells for rapid expression screening and protein production (Robert Novy et al.) 11. Generation of stable CHO cell lines for protein expression (Zhijian Lu et al.) 12. Transient expression in HEK293-EBNA1 cells (Yves Durocher, Roseanne Tom and Louis Bisson) 13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria (Oscar Kuipers and Jan Kok) 14. Protein expression using lentiviral vectors (Bernard Massie, Renald Gilbert and Sophie Broussau) 15. Expression in mammalian cells using BacMam viruses (Yu-Chen Hu and Hsiao-Ping Lee) List of suppliers;Index

Why do children, parents and society seem to be so out of order? The Generational Order process has broken down! This book explains how we got off track, the answers to how we continue to decline, and ultimately, how we have the power to reverse this negative trend. Generational Order is a term coined by Daron Barker to describe the natural law that exists on a primary level that regulates the continuous reoccurring cycles of procreation and manages the acquisition, circulation and preservation of knowledge for the sole purpose of maintaining balance in the natural processes that makes human life successful and progressively better with each generation. In short, when we work with nature, nature works with us, and when we work against nature, nature works against us. Discover the secrets to this ancient law of human interaction that has existed since the beginning. Understand it, use it, spread the word, and restore your family, schools, churches and community! Get your copy today!