Plant Cell Culture Protocols
Title | Plant Cell Culture Protocols PDF eBook |
Author | Víctor M. Loyola-Vargas |
Publisher | Springer Science & Business Media |
Total Pages | 389 |
Release | 2008-02-04 |
Genre | Science |
ISBN | 1592599591 |
A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.
Plant Cell Culture Protocols
Title | Plant Cell Culture Protocols PDF eBook |
Author | Víctor M. Loyola-Vargas |
Publisher | Humana Press |
Total Pages | 430 |
Release | 2012-05-10 |
Genre | Science |
ISBN | 9781617798177 |
Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.
Plant Cell Culture
Title | Plant Cell Culture PDF eBook |
Author | Michael R. Davey |
Publisher | John Wiley & Sons |
Total Pages | 358 |
Release | 2010-05-20 |
Genre | Science |
ISBN | 9780470686515 |
The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.
Plant Tissue Culture
Title | Plant Tissue Culture PDF eBook |
Author | B. N. Sathyanarayana |
Publisher | I. K. International Pvt Ltd |
Total Pages | 299 |
Release | 2007 |
Genre | Botany, Experimental |
ISBN | 8189866117 |
Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.
Plant Cell Culture
Title | Plant Cell Culture PDF eBook |
Author | R. A. Dixon |
Publisher | IRL Press |
Total Pages | 254 |
Release | 1994 |
Genre | Cell culture |
ISBN | 9780199634026 |
Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students.
Plant Cell Culture
Title | Plant Cell Culture PDF eBook |
Author | Hamish A. Collin |
Publisher | Taylor & Francis |
Total Pages | 172 |
Release | 1998 |
Genre | Science |
ISBN |
All the information necessary to set up and run a tissue culture facility is provided in this introductory book.; ; Includes an overview of all the basic tissue culture techniques and describes in detail both the theoretical background and the practical a
Plant Cell, Tissue and Organ Culture
Title | Plant Cell, Tissue and Organ Culture PDF eBook |
Author | Oluf Gamborg |
Publisher | Springer Science & Business Media |
Total Pages | 367 |
Release | 2013-06-29 |
Genre | Science |
ISBN | 3642790488 |
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.