With the interest in biomarkers available for diagnostic, therapeutic, prognostic and research applications growing rapidly, it is now expected that every pathologist should be aware of the correct application of immunohistochemistry in daily practice. This fully updated fourth edition provides practical guidance about preparation, key uses and pitfalls of antibodies for immunohistology in a unique A-Z format. Seventeen full colour infographics present detail about specific biomarkers, including FOXL2, GATA3 and SOX10, pulling out information about expression in tissues and prognosis. Expanded with new biomarkers and the removal of biomarkers that are no longer in common use, each entry in this concise new edition follows a standard structure for quick and easy access by busy readers.

A detailed, A-Z guide and an indispensable source for pathologists ensuring correct application of immunohistochemistry in daily practice.

An updated edition provides a comprehensive list of antisera and monoclonal antibodies that have useful diagnostic applications.

This book provides a comprehensive and up-to-date listing of the sources of immunochemicals for use in laboratory-based specialties such as Histopathology. Much more than just a catalogue of these chemicals, it includes specific technical details on the application and specificities of antisera, common pitfalls in use and how to avoid them, an aspect not covered in other similar texts.

As immunohistochemistry enters its fourth decade as a diagnostic tool, this analytical procedure continues to experience poor reproducibility, albeit in specific situations. The problem becomes more important with the mounting pressure to employ the assay in a quantitative manner for the assessment of therapeutic and prognostic markers in a number of neoplasms. Qualitative applications of immunohistochemistry are well established and continue to increase with the range of sensitive antibodies and detection systems available, however, numerous variables that influence the immunoexpression of proteins in formalin-fixed tissue continue to exist in the pre-analytical and analytical phases of the test procedure. This book emphasises these problems in detail and suggests possible solutions.

The advantages and disadvantages of each immunostaining technique are discussed in this informative and useful text. Immunofluorescence and immunoenzyme staining procedures, including acquisition of reagents, tissue preparation, quality control and methodological trouble shooting, are highlighted. The use of immunohistology for diagnostic evaluation of Kidney diseases, skin diseases, lymphoproliferative diseases, difficult to diagnose neoplastic diseases, nervous system diseases, and infectious diseases is presented. This book is beneficial for diagnostic anatomic pathologists, and biomedical researchers. Chapters Cover: Advantages and disadvantages of each immunostaining technique Acquisition of reagents Tissue preparation Quality control Methodological trouble shooting