Attempts to cover a wide range of both basic research and applied clinical topics related to skeletal muscle damage and repair mechanisms and their application. This book examines muscle damage and repair mechanisms and issues in specific populations including older adults and special populations.

Since the middle of the last century we have progressively built up a comprehensive descriptive model of the allied mechanisms that maintain our muscles at a size and strength appropriate to the functional demands upon them and that rapidly repair damaged muscles. This volume is an assemblage of the collective experience from the pick of major research groups investigating these aspects of muscle cell biology. It provides up-to-date coverage and presents a broad range of topics.

This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system.

Skeletal muscle consumes significant amounts of oxygen, and its oxygen flux increases significantly under conditions of exercise and muscle contraction. This makes the muscle vulnerable to oxidative stress since concomitantly with the increase of oxygen flow there is an increase of free oxygen radicals which are a byproduct of muscle respiration. A number of studies in the last decade have documented the involvement of free oxygen radicals in exercising muscles. The consequences of muscle oxidative stress have resulted mainly in increased muscle protein oxidation, elevation of lipid peroxidation, and depletion of muscle antioxidants. The mechanisms of this oxidative stress are under extensive investigation in laboratories around the world and are topics of the chapters in this volume. This book is intended for professionals who are interested in muscle function, physiology, pathophysiology and well-being, such as therapists, trainers and medical professionals as well as for researchers in the field of muscle physiology.

In order to complete tissue regeneration, various cells (neuronal, skeletal and smooth) interact coordinately with each other. This book, Muscle Cell and Tissue - Current Status of Research Field, deals with current progress and perspectives in a variety of topics on the skeletal and smooth muscle, stem cells, regeneration, disease or therapeutics. Novel applications for cell and tissue engineering including cell therapy, tissue models and disease pathology modeling are introduced. This book also deals with the differentiation/de-differentiation process of vascular smooth muscle cells in health and disease. Furthermore, natural products to reverse metabolic syndromes are descriptively reviewed. These chapters can be interesting for graduate students, teachers, physicians, executives and researchers in the field of molecular biology and regenerative medicine.

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