Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.

The cultivation, isolation and maintenance of rhizobia; Cultivation; Isolation; Maintenance; The qualitative characteristics of rhizobia; Examination with the light microscope; Cultural and metabolic characteristics; Antigenic properties; Bacteriophage and lysogeny; Recognition of rhizobia; Enumeration; Determination of total growth; Counts of viable rhizobia; The assessment of nodulation and nitrogen fixation; Methods for greenhouse and light room; Field trials; Assessment of the need for legume inoculation; The production, control and use of legume inoculants.

General information on the symbiotic nitrogen fixation. Isolation, identification and counting of rhizobia. Production of an inoculant and inoculation of legumes. Experiments.

I. Manipulation of Rhizobia; II. Field and greenhouse assessment of N2 fixation.

This book reviews the history and development of rhizobial ecology (diversity, function and interactions with the biotic and abiotic environments), evolution (genome diversification, systematics of symbiotic genes) and application. Further, it describes the new concept of rhizobia, the latest systematic methods, biogeographic study methods, and genomic studies to identify the interactions between rhizobia, legumes and environments. To enable readers to gain a comprehensive understanding of rhizobial biogeography, the book provides effective protocols for the selection and application of high-efficiency rhizobial inoculants. In addition, it presents standard and modern methods used in studies on rhizobial ecology and evolution in dedicated appendices, making it a unique and valuable handbook for researchers.

This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation.

Sharply focused, up-to-date information on microbial biofertilizers—including emerging options such as Piriformospora indica and Matsutake The Handbook of Microbial Biofertilizers provides in-depth coverage of all major microbial biofertilizers (rhizobia, arbuscular mycorrhizal fungi, and cyanobacteriaas well as new and emerging growth promoters (endophytes). It examines the role of microbes in growth promotion, bioprotectors, and bioremidiators, and presents protocols and practical strategies for using microbes in sustainable agriculture. An abundance of helpful charts, tables, and figures make complex information easy to access and understand. In this first-of-its-kind volume, contributors from 11 countries and several continents address important issues surrounding microbial biofertilizers, including: the rhizobium-host-arbuscular mycorrhizal tripartite relationship mycorrhiza as a disease suppresser and stress reducer mycorrhiza helping bacteria the impact of functional groups of soil microorganisms on nutrient turnover PBPRs as biofertilizers and biopesticides the potential of wild-legume rhizobia for use as a biofertilizers the expanding role of blue-green algae in sustainable agriculture the role of microbial fertilizers in sustainable plant production new and emerging endophytes the commercial potential of biofertilizers In this young century, the use of biofertilizers is already growing rapidly. It has been recognized that these environment-friendly bioprotectors, growth boosters, and remediators are essential for soil/plant health. The Handbook of Microbial Biofertilizers is designed to fit the expanding information needs of current and future biotechnologists, microbiologists, botanists, agronomists, environmentalists, and others whose work involves sustained agriculture.