Fluorescence Microscopy of Living Cells in Culture, Part A

Fluorescence Microscopy of Living Cells in Culture, Part B

Parts A and B, companion volumes on fluorescence microscopy, are sourcebooks of methods for living cells. Part A comprehensively covers methodology: fluorescent analogs, procedures for fluorescent labeling of various cellular structures, and fluorescence microscopy. Part B describes instrumentation and quantitation analysis for imaging and spectroscopy. Key Features * Convenient bench-top format Comprehensive methods inluded for: * Tracing proteins, peptides, hormones, and toxins * Labeling cell surfaces, mitochondria, endoplasmic reticulum, and endocytic compartments * Preparation of fluorescent probes and their incorporation into cells * Culturing cells under the microscope * Basic fluorescence microscopy and imaging techniques

Annotation On preparation, delivery, and detection of fluorescent probes. Discusses factors which determine the destination of probes and methods for delivering them to specific sites in living cells. Includes issues related to sample physiology (microscopic cell culture), optics (basic fluorescence microscopy), and signal detection (electric photometry and imaging, immunoelectron microscopic detection of fluorophores). Annotation copyrighted by Book News, Inc., Portland, OR.