This updated and expanded edition explores key methodologies to study the fascinating phenomenon of how plants readjust their growth toward gravity. In addition to the protocols delivering broad applications for gaining insight into other plant physiological processes, this new volume also focuses on techniques involving plants in space or the use of microgravity analogs to study plant biological phenomenon. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Gravitropism: Methods and Protocols, Second Edition serves as an ideal guide for researchers studying the cellular, molecular, and biochemical networks that plants use to translate environmental stimuli into a growth response.

This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data collection and image analysis; and techniques to investigate ion, organelle, and auxin transporter dynamics, particularly in living cells, as the plant is responding to a change in its orientation. The next few chapters talk about topics that are essential for understanding the complexities underlying tropisms and plant movements in general, and outline basic protocols on handling ornamental flowering shoots for basic plant gravitropism studies. The book concludes with chapters that discuss plant biological studies in space in order to take advantage of unique microgravity conditions not available in Earth-based studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Plant Gravitropism is the perfect book for researchers in the plant scientific community because it is not only useful for plant gravitropism studies, but also addresses a range of interesting problems in plant growth and development.

This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data collection and image analysis; and techniques to investigate ion, organelle, and auxin transporter dynamics, particularly in living cells, as the plant is responding to a change in its orientation. The next few chapters talk about topics that are essential for understanding the complexities underlying tropisms and plant movements in general, and outline basic protocols on handling ornamental flowering shoots for basic plant gravitropism studies. The book concludes with chapters that discuss plant biological studies in space in order to take advantage of unique microgravity conditions not available in Earth-based studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Plant Gravitropism is the perfect book for researchers in the plant scientific community because it is not only useful for plant gravitropism studies, but also addresses a range of interesting problems in plant growth and development.

Tropisms, the defined vectorial stimuli, such as gravity, light, touch, humidity gradients, ions, oxygen, and temperature, which provide guidance for plant organ growth, is a rapidly growing and changing field. The last few years have witnessed a true renaissance in the analysis of tropisms. As such the conception of tropisms has changed from being seen as a group of simple laboratory curiosities to their recognition as important tools/phenotypes with which to decipher basic cell biological processes that are essential to plant growth and development. Plant Tropisms will provide a comprehensive, yet integrated volume of the current state of knowledge on the molecular and cell biological processes that govern plant tropisms.

This book summarizes what is currently known about gravity sensing and response mechanisms in microorganisms, fungi, lower and higher plants; starting from the historical eye-opening experiments from the 19th century up to today’s extremely rapid advancing cellular, molecular and biotechnological research. All forms of life are constantly exposed to gravity and it can be assumed that almost all organisms have developed sensors and respond in one way or the other to the unidirectional acceleration force,this books shows us some of these different ways. The book is written for plant biologists and microbiologists as well as scientists interested in space and gravitational biology.

Plants provide a source of survival for all life on this planet. They are able to capture solar energy and convert it into food, feed, wood and medicines. Though sessile in nature, over many millions of years, plants have diversified and evolved from lower to higher life forms, spreading from sea level to mountains, and adapting to different ecozones. They have learnt to cope with challenging environmental conditions and various abiotic and biotic factors. Plants have also developed systems for monitoring the changing environment and efficiently utilizing resources for growth, flowering and reproduction, as well as mechanisms to counter the impact of pests and diseases and to communicate with other biological systems, like microbes and insects. This book discusses the “awareness” of plants and their ability to gather information through the perception of environmental cues, such as light, gravity, water, nutrients, touch and sound, and stresses. It also explores plants’ biochemical and molecular “computing” of the information to adjust their physiology and development to the advantage of the species. Further, it examines how plants communicate between their different organs and with other organisms, as well as the concepts of plant cognition, experience and memory, from both scientific and philosophical perspectives. Lastly, it addresses the phenomenon of death in plants. The epilogue presents an artist’s view of the beauty of the natural world, especially plant “architecture”. The book provides historical perspectives, comparisons with animal systems where needed, and general biochemical and molecular concepts and themes. Each chapter is selfcontained, but also includes cross talk with other chapters to offer an integrated view of plant life and allow readers to appreciate and admire the functioning of plant life from within and without. The book is a tribute by the Editor to his students, colleagues and co-workers and to those in whose labs he has worked.

The current growing interest of molecular biologists in plant hormone research is undoubtedly the most promising development of recent times. Many papers were presented during the 14th International Conference on Plant Growth Substances illustrating the impact of this new approach on our understanding of hormone-controlled processes. The specific character is the integrated study of plant growth regulation at all levels ranging from single molecules to the entire plant and its functioning in the environment. Hormones play an essential role in the regulation, but not an exclusive one. Other compounds and factors, such as Ca2+, for instance are often of equal relevance, because they may take part in the signal transduction pathway. Moreover, regulation of the regulator by non-hormonal factors is an essential part of any control mechanism. The present volume reflects the change in interest from plant growth substances to plant growth regulation.