Raman microscopy is now well-established as one of the most powerful and versatile techniques for a diverse range of applications in both research and analytical laboratories. Its unique advantage is its ability to noninvasively characterize chemically complex and spatially inhomogeneous samples with a sub-micron spatial resolution. Modern confocal Raman scanning microscopy, which allows one to obtain two- and three-dimensional spectrochemical images of samples in various states and forms, has become a method of choice for a wide range of applications including the study of biological cells, tissues, and microorganisms, characterization of pharmaceutical drugs and formulations, forensic evidence, minerals and gems, carbon nanomaterials, semiconductors, composite polymers, and more. This book presents the techniques of confocal Raman microscopy and imaging for researchers and engineers from a variety of disciplines. It highlights the key aspects of this technique in order to effectively apply it in practice. It will appeal to a wide circle of readers who are interested in, or are already, using the methods of confocal Raman microscopy and imaging in their work, and will also be beneficial for novice Raman microscopy users.

This book reflects the dramatic increase in the number of Raman spectrometers being sold to and used by non-expert practitioners. It contains coverage of Resonance Raman and SERS, two hot areas of Raman, in a form suitable for the non-expert. Builds Raman theory up in stages without overloading the reader with complex theory Includes two chapters on instrumentation and interpretation that shows how Raman spectra can be obtained and interpreted Explains the potential of using Raman spectroscopy in a wide variety of applications Includes detailed, but concise information and worked examples

Second edition of the guide to the modern techniques that demonstrate the potential of Raman spectroscopy Completely revised and updated, the second edition of Modern Raman Spectroscopy presents the information needed for clear understanding and application of the technique of Raman Spectroscopy in a range of areas such as pharmaceuticals, forensics, and biology. The authors—noted experts on the topic—reveal how to make full use of the critical information presented and include a wealth of examples of the pitfalls that can be encountered. The text opens with a description of the basic theory to assist readers in making a practical interpretation of Raman Spectra. Chapters include the main equations that are used in order to highlight the theory’s meaning and relevance while avoiding a full mathematical treatment. Modern Raman Spectroscopy provides a firm grounding, combined with a variety of references, from which to approach a more comprehensive study of specific aspects of Raman Spectroscopy. This new edition: Includes instrumentation sections that now contain Spatially Offset Raman scattering and transmission Raman scattering Offers an updated SERS chapter that presents recent examples and Tip enhanced Raman scattering Contains updated information with an emphasis on pharmaceutical, forensic, and biological applications Introduces modern techniques in the imaging and mapping of biological samples and more advanced methods which are becoming easier to use Written for users of Raman Spectroscopy in industry, including non-analysts, researchers, and academics, the second edition of Modern Raman Spectroscopy clearly demonstrates the potential of using Raman Spectroscopy for a wide range of applications.

This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy. The book includes expanded background information and adds insights into how confocal Raman microscopy, especially 3D Raman imaging, can be integrated with other methods to produce a variety of correlative microscopy combinations. The benefits are then demonstrated and supported by numerous examples from the fields of materials science, 2D materials, the life sciences, pharmaceutical research and development, as well as the geosciences.

Raman spectroscopy is now well established as one of the most versatile techniques for the chemical analysis of molecular species. Major advances have been made in a number of areas in the field in recent years which enable the researcher and practising analytical scientist to solve the complex chemical problems of today. The ten chapters in Modern Techniques in Raman Spectroscopy cover some of the most exciting fields of research in modern Raman techniques, and illustrate the power of modern Raman spectroscopy for molecular analysis in both theoretical and practical problems. The volume opens with chapters on signal expressions and instrumentation in Raman spectroscopy, and then goes on to discuss in detail Fourier and Hadamard Transform Raman spectroscopies, micro-Raman spectroscopy, surface-enhanced Raman spectroscopy, Raman optical activity, coherent and time-resolved techniques and the use of optical fibres in Raman spectroscopy. The chapters are written by leading researchers from a broad range of disciplines. Throughout, applications of the various techniques are discussed. Modern Techniques in Raman Spectroscopy will be of great interest to all those involved in molecular spectroscopy, in both industry and academia. The inclusion of a wide range of modern techniques in a single volume will make this a particularly valuable work to researchers across the whole field of Raman spectroscopy.

Minimaler Aufwand bei der Probenvorbereitung, hoher Informationsgehalt des Spektrums und die Möglichkeit, mit festen Proben zu arbeiten, machen die Raman-Spektroskopie zunehmend attraktiv. Wie man diese Methode mit modernster Ausrüstung effizient anwendet, zeigt Ihnen das vorliegende Buch. Im Mittelpunkt stehen neue Entwicklungen wie CCDs, Diodenlaser und Fourier-Transform-Techniken. Behandelt werden auch quantitative Analysen, die in der bisher vorhandenen Literatur häufig zu kurz kamen. (08/00)

Modern Vibrational Spectroscopy and Micro-Spectroscopy: Theory, Instrumentation and Biomedical Applications unites the theory and background of conventional vibrational spectroscopy with the principles of microspectroscopy. It starts with basic theory as it applies to small molecules and then expands it to include the large biomolecules which are the main topic of the book with an emphasis on practical experiments, results analysis and medical and diagnostic applications. This book is unique in that it addresses both the parent spectroscopy and the microspectroscopic aspects in one volume. Part I covers the basic theory, principles and instrumentation of classical vibrational, infrared and Raman spectroscopy. It is aimed at researchers with a background in chemistry and physics, and is presented at the level suitable for first year graduate students. The latter half of Part I is devoted to more novel subjects in vibrational spectroscopy, such as resonance and non-linear Raman effects, vibrational optical activity, time resolved spectroscopy and computational methods. Thus, Part 1 represents a short course into modern vibrational spectroscopy. Part II is devoted in its entirety to applications of vibrational spectroscopic techniques to biophysical and bio-structural research, and the more recent extension of vibrational spectroscopy to microscopic data acquisition. Vibrational microscopy (or microspectroscopy) has opened entirely new avenues toward applications in the biomedical sciences, and has created new research fields collectively referred to as Spectral Cytopathology (SCP) and Spectral Histopathology (SHP). In order to fully exploit the information contained in the micro-spectral datasets, methods of multivariate analysis need to be employed. These methods, along with representative results of both SCP and SHP are presented and discussed in detail in Part II.