This volume is based on prec'entations at the conference on Culture of Marine Invertebrate Animals which was held in Green port, New York in October, 1972. The conference was sponsored by the Middle Atlantic Natural Sciences Council, Inc., a non profit educational corporation, together with the Marine Science Centers of Adelphi University, the State University of New York at Stony Brook, Long Island University, Suffolk County Community College, and the Shelter Island Oyster Company. The purpose of the conference was to provide a needed ex change of knowledge among scientists of various specialties whose information would be invaluable to others confronted with similar problems, even with different marine animals. Part I considers supportive techniques -- general isolation and culture methods, problems of disease and feeding. Specific techniques employed in the culture of a wide range of invertebrate organisms is covered in Part II. We want to thank the contributors for their cooperation in preparing the manuscripts based on their conference presentations. Walter L. Smith Matoira H. Chanley v Contents PART I Recirculating System Culture Methods for Marine Organisms .............•.

Biological filtration; Mechanical filtration; Physical adsorption; Disinfection; Gas exchange and respiration; Seawater; Buffering; Toxicity and disease prevention; Analytical methods.

This reference work is designed to provide background information on an array of northeastern Pacific marine invertebrate species so that they can be more easily included in comparative studies of morphology, cell biology, reproduction, embryology, larval biology, and ecology. It is meant to serve biologists who are new to the field as well as experienced investigators who may not be familiar with the invertebrate fauna of the northern Pacific Coast. The species discussed in this volume are mostly from the cold temperate waters of the San Juan Archipelago, near Puget SOund and the Strait of Georgia, but the information and methods given will be useful in laboratories from Alaska to central California and applicable to some extend in other coastal or inland facilities. An introductory chapter discusses basic prodcedures for collecting and maintaining mature specimens, for initiating spawning, and for culturing embryos and larvae in the laboratory. Subsequent chapters summarize reproduction and development in thirty different invertebrate groups and provided ercent references through which additional information can be traced, cite monographs or keys needed to identify species, and give methods useful for studying an array of selected species. Available information on habitat, diet, reproductive mode, egg size, developmental pattern, developmental times, larval type, and conditions for settlement and metamorphosis is reported for over 450 species.