A hiking and exploring guidebook to the areas that surround Bend, Oregon. Features hikes, hot springs, canyons, caves, waterfalls, lakes, and lavalands.

Born on the eve of China’s Cultural Revolution, Ping Fu was separated from her family at the age of eight. She grew up fighting hunger and humiliation and shielding her younger sister from the teenagers in Mao’s Red Guard. At twenty-five, she found her way to the United States; her only resources were $80 and a few phrases of English. Yet Ping persevered, and the hard-won lessons of her childhood guided her to success in her new homeland. Aided by her well-honed survival instincts, a few good friends, and the kindness of strangers, she grew into someone she never thought she’d be—a strong, independent, entrepreneurial leader. “She tells her story with intelligence, verve and a candor that is often heart-rending.” —The Wall Street Journal “This well-written tale of courage, compassion, and undaunted curiosity reveals the life of a genuine hero.” —Booklist (starred review) “Her success at the American Dream is a real triumph.” —The New York Post

Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins. Many cellular processes involve special proteins that bind to specific DNA sites with high affinity. How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DNA at specific sites and indicate that DNA deformability is a key factor in site-specific recognition. However, the dynamics of DNA deformations have been difficult to capture, thus obscuring our understanding of recognition mechanisms. The experiments presented in this thesis uncover, for the first time, rapid (~100-500 microseconds) DNA unwinding/bending attributed to nonspecific interrogation, prior to slower (~5-50 milliseconds) DNA kinking/bending/nucleotide-flipping during recognition. These results help illuminate how a searching protein interrogates DNA deformability and eventually “stumbles” upon its target site. Submillisecond interrogation may promote preferential stalling of the rapidly scanning protein at cognate sites, thus enabling site-recognition. Such multi-step search-interrogation-recognition processes through dynamic conformational changes may well be common to the recognition mechanisms for diverse DNA-binding proteins.